Applied biosystems 7500 fast, 7500 and 7300 realtime pcr. Interact with the latest advancements in touch screen usability, access your. Every consumable run on a thermo fisher scientific realtime qpcr instrument requires the creation of one or more experiment files that store the associated data. Prior to downloading any software to a computer used for instrument controloperation, please consult with your service and support. The applied biosystems 7500 realtime pcr system is a 3rd generation of thermocycling technology that allows quanitation of product generation in real time.
Disclaimer the text above is not a recommendation to uninstall realtime pcr software v2. Applied biosystems viia 7 realtime pcr system thermo. The 7500 realtime pcr system is a powerful platform for labs requiring superior performance and maximum dye versatility. The applied biosystems analysis software can import and analyze data generated by any of the supported instruments listed in the following table. The applied biosystems analysis software is a secure web application for analysis of data that are generated on thermo fisher scientific realtime pcr instruments. Applied biosystems stepone and steponeplus realtime pcr systems remarkably simple systems. Applied biosystems 7500 getting started pdf download.
With our applied biosystems realtime pcr platforms, you get true value with excellent performance, reliability, and worldclass support. Researchers use applied biosystems integrated systems for sequencing, flow cytometry, and realtime, digital and end point pcrfrom sample prep to data analysis. Proven performance and superior data quality at an excellent value the 7300 real time pcr system balances exceptional quality with budget sensitivity. The plexor hy systemaf is a novel realtime pcr system for quantification of total human autosomal and human male y dna within a sample. This applied biosystem prism 7000 sequence detection system is in excellent condition. Perform standard pcr in less than 2 hours and fast pcr reactions in less than 40 minutes intuitive, flexible software and wizards guide new users through. Which version of the applied biosystems 7500 realtime pcr.
Applied biosystems 7500 fast realtime pcr system, laptop. Applied biosystems 7900ht fast realtime pcr system and sds. These 96 well realtime pcr systems offers four color optical recording allowing for faster measurement. This system is a sophisticated platform for users who require extended capabilities and maximum versatility. Applied biosystems 7500 realtime pcr system, laptop. Utilizing robust led based 3color optical recording, the stepone realtime pcr system is designed to deliver precise, quantitative realtime pcr results for a variety of genomic research applications. The 7300 is a very reliable platform with a high quality peltierbased heating block providing uniform heating and cooling of all the wells. The abi 7500 is a real time pcr that delivers accurate results in less than 30 minutes. Feb 24, 2015 the abi 7500 is a real time pcr that delivers accurate results in less than 30 minutes. The applied biosystems analysis software can import and analyze experiment files eds and sds that are generated by various thermo fisher scientific realtime qpcr instruments. Help from within applied biosystems 75007500 fast realtime pcr software v2.
The information on this page is only about version 2. This instrument is capable of quantitative detection using realtime analysis. The applied biosystems high resolution melting hrm software is the easiest to use melting analysis software enabling realtime pcr melt curve assays to be used more accurately for mutation scanning and genotyping. The overall size and footprint of the abi 7500 was made more compact. This system is a sophisticated platform for users who. The software versions listed in the table represent only those tested for use with the applied biosystems software. Applied biosystems 7500 realtime pcr system software version 2. Applied biosystems 7500 fast, 7500 and 7300 realtime pcr systems. The applied biosystems 7300 real time pcr system is an economical solution setting the standard for labs requiring a quality platform at an affordable price. Applied biosystems 75007500 fast realtime pcr system getting.
Realtime pcr system supported software versions file extension applied biosystems 7900 ht fast realtime pcr system v2. Applied biosystems 75007500 fast realtime pcr system getting started guide for relative standard curve and comparative c t experiments 4387783 applied biosystems 7900ht fast realtime pcr system relative quantitation using comparative c t getting started guide 4364016 applied biosystems stepone and steponeplus realtime pcr. The 7500 fast dx realtime pcr instrument delivers the performance required for highquality results in a 96well format. The abi prism 7000 sequence detection system qpcr rtpcr 7000 sds instrument is a secondgeneration sequence detection instrument capable of quantitative and qualitative detection with fluorescentbased pcr chemistries. G161544 applied biosystems 4376592 steponeplus realtime pcr. These factorycalibrated onestep systems make it easy to step up to highperformance qpcr.
Real time pcr model applied biosystems 7500 now on sale at the lab world group. Applied biosystems instruments, assays, and reagents have been trusted in the lab for over 20 years. Applied biosystems realtime pcr instruments thermo. Plexor hy system for the applied biosystems 7500 and 7500. The 7500 real time pcr system is a powerful platform for labs requiring superior performance and maximum dye versatility. Laboratory equipment measuring instruments bicycle accessories software control unit. The stepone and steponeplus systems software contains. The hrm software is available on the 7500 fast realtime pcr system, which delivers precise results in a standard 96well. An example of data from the desktop software is shown by a maize genotyping experiment. Perform standard pcr in less than 2 hours and fast pcr reactions in less than 40 minutes intuitive, flexible software. Features of the the stepone realtime pcr system include.
Applied biosystems abi 7500 fast realtime pcr system. May 10, 2005 the applied biosystems 7500 realtime pcr system is a 3rd generation of thermocycling technology that allows quanitation of product generation in real time. View online or download applied biosystems 7500 installation and maintenance manual, getting started, getting started manual. The abi 7500 is the predecessor of the abi 5700, made as the lower throughput version of the abi 7900ht. Applied biosystems stepone steponeplus realtime pcr systems.
Applied biosystems 7500 realtime pcr system, laptop fisher. The applied biosystems 7500 is todays top choice in rt pcr. Relative quantitation rq algorithms in applied biosystems realtime pcr systems software. Designed for compatibility with applied biosystems taqman assays and the flexibility of unlimited realtime chemistry choices, we make it easy for you to get started with your experiments.
Feb 22, 2016 real time pcr model applied biosystems 7500 now on sale at the lab world group. This technical manual describes the plexor hy system and provides instructions for use with the applied biosystems 7500 and 7500 fast realtime pcr systems, including reaction setup, realtime. A tutorial, using example experiment data provided with the applied biosystems stepone realtime pcr software stepone software. Applied biosystems, a leader in technology solutions for life science research, introduces its latest innovations in realtime pcrthe stepone 48well and steponeplus. Applied biosystems steponetm and steponeplustm realtime pcr systems site preparation guide 7 preface obtaining information from the help system the stepone software has a help system that.
The applied biosystems 7500 realtime pcr system is a 96well, fivecolor platform that uses fluorescencebased polymerase chain reaction pcr reagents to analyze items. The applied biosystems stepone and steponeplus realtime pcr systems offer intuitive software, fast, and are configured for pcfree or networked operation. Prior to downloading any software to a computer used for instrument controloperation, please consult with your service and support representative. In no event shall applied biosystems be liable for incidental, special, multiple, or consequential damages in connection with or arising from the use of this document. Intended for laboratory staff and principal investigators who perform experiments using the stepone or steponeplus system. The software provides projectbased analysis of realtime and endpoint data for various quantitative and qualitative pcr applicatio. Watch the video, find your best fit, or request a quote. This real time pcr system combines pre boxed or custom assays, and advanced thermal cycling systems with responsive software. The applied biosystems realtime pcr software runs off of windows xp platform and even works with windows 7.
Applied biosystems abi 7500 real time pcr video youtube. Application note quantstudio 3 and 5 realtime pcr systems. Realtime pcr videos scientific videos thermo fisher. Specifically designed with a user friendly, yet powerful interface, they are ideal for both new and experienced realtime pcr researchers. Applied biosystems relative quantitation analysis module.
The hrm software is available on the 7500 fast realtime pcr system, which delivers precise results in a standard 96well format. The applied biosystems steponeplus realtime pcr system, makes it simple and easy to get highquality realtime pcr results. Applied biosystems steponeplus realtime pcr system, laptop. Prior to downloading any software to a computer used for instrument controloperation, please. Field application specialist doug rains offers advice for fixing common software setup. Applied biosystems stepone realtime pcr software stepone software. This real time pcr system combines pre boxed or custom assays, and advanced thermal cycling systems with responsive software for a vast array of applications. Notes applied biosystems 75007500 fast realtime pcr system getting started guide for standard curve experiments. Applied biosystems 7500 realtime pcr system biocompare. Field application specialist doug rains offers advice for fixing common software setup mistakes when performing realtime pcr. G161544 applied biosystems 4376592 steponeplus realtime. Applied biosystems pioneered the development of realtime pcr in 1995, enabling highthroughput, quantitative gene expression.
May 11, 2009 to achieve high quality results of qpcr, a superb apparatus is needed and this is where you can turn to the applied biosystems 7300 real time pcr system. Applied biosystems quantstudio 12k flex realtime pcr system applied biosystems quantstudio 1 realtime. The software provides projectbased analysis of realtime and endpoint data for various quantitative and qualitative pcr applications. The steponeplus realtime pcr system can be used via a pc, with a pc connected to a local area network lan, or as a standalone instrument pcfree. The steponeplus can even assist you with selecting and ordering pcr reagents needed to run your experiment. The applied biosystems stepone and steponeplus realtime pcr systems offer intuitive software, fast and standard runs, and are configured for pcfree or networked operation. Applied biosystems steponeplus realtime pcr system. Applied biosystems stepone steponeplus realtime pcr. Fixing software setup mistakes in realtime pcr setponeplus asktaqman ep 10. Applied biosystems 7500 realtime pcr system thermo fisher. Each steponeplus realtime pcr system is factorycalibrated for optical and thermal accuracy, so simply remarkable realtime pcr results can be obtained right out of the box. Advanced software and instrumentation for performing a wide array of genomic assays. Applied biosystems shown were made automatically by the desktop software. Top sites applied biosystems real time pcr 2019 latest.
Applied biosystems 7500 fast realtime pcr system thermo. Researchers use applied biosystems integrated systems for sequencing, flow cytometry, and real. Applied biosystems 7900ht fast realtime pcr system and. This remarkably simple realtime pcr system is designed with an lcd touchscreen and a userfriendly interface that brings the power of genetic studies to researchers new to realtime pcr fig 1.
The applied biosystems steponeplus is a realtime pcr system designed to meet your needs. Applied biosystems realtime pcr rapid assay development guidelines are a series of design and experimental guidelines aimed towards maximizing success while reducing upfront time and costs of. Which version of the applied biosystems 7500 realtime pcr system software can be used to run the investigator. Applied biosystems stepone realtime pcr system fisher. Check out the most popular applied biosystems products for genetic analysis. Primerdesign ltd covid19 genesig realtime pcr assay. User bulletin applied biosystems realtime pcr systems july 2007 subject. Applied biosystems stepone and steponeplus realtime pcr systems getting started guide for 23 presenceabsence experiments notes. These factorycalibrated systems make it easy to step up to highperformance qpcr. The applied biosystems 7500 fast real time pcr offers an intuative and flexible solution for rapid detection and quantification of nucleic acids. Steponeplus realtime pcr systems getting started guide for genotyping experiments explains how to perform experiments on the stepone and steponeplus systems. The calibrate function is also available in the control console. Applied biosystems realtime pcr rapid assay development.