The streptozotocin diabetic rat as a model of the chronic complications of human diabetes michael wei1 bsc, leslie ong1 bsc, maree t. Standard operating procedure for streptozotocin stz in. Streptozocin pharmacokinetics absorption bioavailability. Guidelines on use of streptozotocin in rodents ulam. Mode of action of streptozotocin journal of bacteriology asm. The mechanism of alloxan and streptozotocin action in b cells of the rat pancreas t. Tevas generic of zanosar streptozocin sterile powder. Streptozotocin diabetes is caused by the specific necrosis of the pancreatic. Burr department of pediatrics, vanderbilt university medical school, nashville, tennessee, usa summary. Streptozotocin stz is commonly used to induce experimental diabetes in animals. Full text streptozotocininduced type 1 diabetes in. Streptozotocin is an antimicrobial agent and has also been used as a chemotherapeutic alkylating agent. Hoey4 phd, darryl burstow5 mbbs fracp and lindsay brown1 phd departments of physiology and pharmacology1 and pharmacy2, the university of queensland. Alloxan and streptozotocin are toxic glucose analogues that preferentially accumulate in pancreatic beta cells via the glut2 glucose transporter.
Manufacturing cmc label is not available on this site. Pdf streptozotocinnicotinamideinduced rat model of. The catalog displays all strengths and sizes along with the description, imprint code, ndc and photo for each product. Evaluation of antidiabetic activity of bambusa vulgaris. The beta cell toxic action of alloxan is initiated by free radicals formed in. The decrease in nad is due to increased nad degradation. Jul 25, 2019 streptozocin can pass into body fluids urine, feces, vomit. Streptozocin comes as a powder to be mixed with liquid and given intravenously into a vein by a doctor or nurse in a medical facility. Streptozocin was used to induce type 1 diabetes tocris bioscience is the leading supplier of novel and exclusive tools for life science research with over 30 years experience in the industry. Superoxide dismutase was administered intravenously to rats 50min prior to. Sigmaaldrich offers a number of streptozocin products. Streptozotocin je organsko jedinjenje, koje sadrzi 8 atoma ugljenika i ima molekulsku masu od 265,221 da. Dec 18, 2007 alloxan and streptozotocin are toxic glucose analogues that preferentially accumulate in pancreatic beta cells via the glut2 glucose transporter. Group 4 was injected with streptozotocin and later treated in the same way as in group 3 streptozotocinmanganese group.
It is used as an antineoplastic agent and to induce diabetes in experimental animals. Streptozocin has been reported to prolong the elimination halflife of doxorubicin and may lead to severe bone marrow suppression. Hoey4 phd, darryl burstow5 mbbs fracp and lindsay brown1 phd departments of physiology and pharmacology1 and pharmacy2, the university of. Streptozocin used on glut2 positive neuroendocrine tumor cells. Protection against streptozotocininduced diabetes by.
An antibiotic, c8h15n3o7, produced by an actinomycete, used primarily to treat pancreatic tumors affecting the islets of langerhans. Get a printable copy pdf file of the complete article 1. Dityrosine formationthe effect of stz on cysteine oxidation. Streptozotocin induced pancreatic injury is commonly used for creating rodent models of type 1 diabetes which develop renal injury with similarities to human diabetic nephropathy. Alkylation of dna in rat tissues following administration of streptozotocin.
Following iv or intraperitoneal administration in animals, rapidly distributed into liver, kidneys, intestine, and pancreas. Cage bedding is considered contaminated until at least 3 days after stz is no longer administered and must be disposed of in the following manner 5. An antibiotic, c 8 h 15 n 3 o 7, produced by an actinomycete streptomyces achromogenes, used primarily to treat pancreatic tumors affecting the islets of langerhans. Streptozotocin induces dna strand breaks and dna alkylation that leads to necrosis of pancreatic. The streptozotocindiabetic rat as a model of the chronic complications of human diabetes michael wei1 bsc, leslie ong1 bsc, maree t. A pancreatic mode of action is feasible because in mild diabetes not all the beta. Google scholar suzuki h, nagai k, yamaki h, tanaka n, umezawa h. Pdf antihyperglycaemic and mode of action of thaumatococcus. Differences in the action of nitrosomethylurea and streptozotocin.
Zanosar may demonstrate additive toxicity when used in combination with other cytotoxic drugs. Streptozotocin or streptozocin inn, usp stz is a naturally occurring alkylating antineoplastic agent that is particularly toxic to the insulinproducing beta cells of the pancreas in mammals. The cytotoxic action of both these diabetogenic agents is mediated by reactive oxygen species, however, the source of their generation is different in the case of alloxan and streptozotocin. Health hazards streptozotocin is an antibiotic that is produced by stretomyces achromogenes, it is a toxic and carcinogenic material. Streptozotocin and alloxan induce dna strand breaks and polyadpribose synthetase in pancreatic islets.
Group 4 was injected with streptozotocin and later treated in the same way as in group 3 streptozotocin manganese group. The precise molecular mechanism of stz cytotoxicity is however not clear. Streptozotocin definition of streptozotocin by the free. Caregivers should wear rubber gloves while cleaning up a patients body fluids, handling contaminated trash or laundry or changing diapers. For at least 48 hours after you receive a dose, avoid allowing your body fluids to come into contact with your hands or other surfaces. Antidiabetic, bambusa vulgaris, glibenclamide, streptozotocin. Protection against streptozotocin induced diabetes by superoxide dismutase m. The possibility is discussed that interaction of streptozotocin with cytosine residues in cellular.
Streptozotocin a diabetogenic agent in animal models ijppr. Protection against streptozotocininduced diabetes by superoxide dismutase m. Streptozocin also known as streptozotocin is an agent with specific effects on pancreatic beta cells. Streptozocin is an nnitrosourea that is an antibiotic produced by streptomyces achromogenes. Explore information about air pollutants, contaminants and studies being done. Superoxide dismutase was administered intravenously to rats 50min prior to intravenous. Feb 06, 2020 get emergency medical help if you have signs of an allergic reaction. In the presence of intracellular thiols, especially glutathione, alloxan generates reactive oxygen species ros in a cyclic redox reaction with its reduction product, dialuric acid. Streptozotocin stimulates the ion channel trpa1 directly. Review of the mechanism of cell death resulting from streptozotocin. Studies on the diabetogenic action of streptozotocin nsc.
The mechanisms of alloxan and streptozotocininduced diabetes. Streptozotocin, a nnitrosocontaining compound that induces death of insulinsecreting cells, producing an animal model of diabetes. Weigh the appropriate amount of stz so your final concentration in the nacitrate buffer. The mechanism of alloxan and streptozotocin action in b. Alloxan and the product of its reduction, dialuric acid, establish a redox cycle with the formation of superoxide radicals. Streptozotocin does not cross the bloodbrain barrier, but its metabolites are found in cerebral spinal fluid. Autoxidation of dialuric acid generates superoxide radicals. Group 3 was given the vehicle for streptozotocin and saline for 1 wk, then received manganese for 2 wk salinemanganese group. Non insulin dependent diabetes mellitus niddm was induced by a single intraperitoneal injection of streptozotocin 60mgkg and nicotinamide 120mgkg to rats pellegrino et al. Cytotoxic to neuroendocrine tumor cell lines that express the glut2 glucose transporter. The length of treatment depends on how well your body responds to. Aug 23, 2018 please visit guidelines on use of streptozotocin in rodents to locate the document you need.
Standard operating procedure for streptozotocin stz in animals 1. In mammalian cells, the mechanism of action of stz that results in cell death was. Identification of the substancemixture and of the companyundertaking product name. This barcode number lets you verify that youre getting exactly the right version or edition of a book.
Benth ethanol leave extract in streptozotocininduced diabetic rats. Handle all cages in a certified chemical fume hood or type ii b2 bsc. Jul 03, 2017 zanosar may demonstrate additive toxicity when used in combination with other cytotoxic drugs. Streptozotocin streptozocin, stz is a naturally occurring chemical that is particularly toxic to the insulinproducing beta cells of the pancreas in mammals. Mode of action of stz cellular toxicity includes its selective. Streptozotocin is a pancreatic betacellspecific cytotoxin and is widely used to induce experimental type 1 diabetes in rodent models. The mechanism of alloxan and streptozotocin action in b cells of the rat pancreas. Papich dvm, ms, dacvcp, in saunders handbook of veterinary drugs fourth edition, 2016. Elucidation of molecular mechanisms of streptozotocin. Bennett and pegg 1981 alkylation of dna in rat tissues following administration of streptozotocin. It is used in medicine for treating certain cancers of the islets of langerhans and used in medical research to produce an animal model for hyperglycemia and alzheimers in a large dose, as well as. Streptozotocin use in mice and rats version 1 date of publication.
Get emergency medical help if you have signs of an allergic reaction. It has a role as an antineoplastic agent, an antimicrobial agent, a dna synthesis inhibitor and a metabolite. Because pancreatic beta cells have high concentrations of glucose transporter 2 glut2, streptozocin is selectively toxic to these cells. The product catalog provides you with a full listing of tevas brand and generic product lines. Streptozotocininduced pancreatic injury is commonly used for creating rodent models of type 1 diabetes which develop renal injury with similarities to human diabetic nephropathy. Streptozotocin 2deoxy23methyl3nitrosoureidod glukopyranose, an antibiotic with antineoplastic effects produced by streptomyces achromogenes bacteria, destroys selectively the. Streptozotocin specifically targets beta cells, entering via the glucose transporter glut2 and causing alkylation of dna. Preparation, administration, handling and care of streptozotocin stz revision no. Group 2 was given streptozotocin and treated with saline for 2 wk streptozotocin group. Please visit guidelines on use of streptozotocin in rodents to locate the document you need. Studies have suggested that stz is preferably absorbed by insulinsecreting. As part of the study into the metabolism and mode of action of the diabetogenic drug streptozotocin, we have reported 1 the synthesis of three specifically. It may be injected once a day for 5 days in row every 6 weeks or it may be injected once a week. Streptozotocin definition of streptozotocin by medical.